Genetic Variation of Lime (Citrus sp.) Accessions Using Flow Cytometry Technique, Morphological Characteristics and Molecular Markers

Authors

  • Behrouz Golein Associate and Assistant Professor of Citrus and Sub Tropical Fruits Institute, Horticultural Science Research Institute, AREEO, Ramsar, Mazandaran, Iran, Respectively.
  • Hamed Hassanzadeh Khankahdani The Former Ph.D Student of Fruit Trees Physiology and Breeding, Horticulture Department of Agricultural and Natural Resource Faculty, Hormozgan University and Horticulture Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Bandar Abbas, Hormozgan, Iran.
  • Morteza Golmohammadi Associate and Assistant Professor of Citrus and Sub Tropical Fruits Institute, Horticultural Science Research Institute, AREEO, Ramsar, Mazandaran, Iran, Respectively.
  • Somayeh Rastegar Assistant Professor of Horticulture Department of Agricultural and Natural Resource Faculty, Hormozgan University, Iran.
Abstract:

Because of sexual propagation of Mexican lime in southern regions of Iran, there are many lime accessions (lime biotypes) in Mexican lime gardens in which appear some variation in fruit and tree shape. However, most of these accessions are susceptible to witches broom disease of lime (WBDL). Persian lime (C. latifolia Tanaka) is a triploid WBDL-tolerant species. Considerable number of un-described lime biotypes have been cultivated by farmers as Persian lime with no documented evidence related to their origin and genetic background. To unveil systematic modality of the lime accessions, we investigated 18 lime accessions collected from Fars, Hormozgan and Mazandaran provinces (Iran) using flow cytometry (FCM) approach, morphological characteristics, ISSR and SSR markers in 2016. The results of FCM revealed that 14 out of 18 lime accessions were triploid. Triploid accessions showed higher leaf dimension values together with lower stomata and secretory sacs density compared with diploid accessions. Application of SSR markers confirmed the obtained results of ploidy level determination using FCM and morphological analysis. Results of morphological and ISSR markers demonstrated that four out of 18 accessions were different from others and it was in correspondence with the results of FCM. SSR results also grouped the accessions in line of FCM. In conclusion, it has been shown that IFJKh, Cucumber-shaped lime and IFJKMes accessions, are diploid and their cultivation is not recommended in the infected regions to WBDL phytoplasma.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Study of genetic diversities and relatedness of Iranian citrus genotypes using morphological and molecular markers

Having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. In present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and RAPD markers. Significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...

full text

Identification of Genetic Variation of Male and Female Date Palm (Phoenix dactylifera L.) Cultivars Using Morphological and Molecular Markers

Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR ampli...

full text

Genetic Variation Pattern of Iranian Wheat Landraces Based on ISSR Molecular Markers and Morphological Traits

Collection and conservation of germplasm, particularly for landraces of important agronomically species and organizing their information, cause to continuity of their usefulness and reduce the risk of their genetic erosion. In this experiment, 10 Iranian wheat landraces along with the Chinese Spring cultivar, as control, were used to assess inter and intra populations variation by using ISSR mo...

full text

study of genetic diversities and relatedness of iranian citrus genotypes using morphological and molecular markers

having knowledge about genetic relationships among accessions is necessary for developing breeding strategies to produce improved cultivars. in present study, genetic diversity and inter-relationship among 29 genotypes of citrus were comparatively analyzed using morphological and rapd markers. significant variability was observed among citrus genotypes for 61 quantitative and qualitative morpho...

full text

Assessment of Genetic Structure and Variation of Native Berberis Populations of Khorasan Provinces (Iran) Using AFLP Markers Versus Morphological Markers

Seedless barberry (Berberis vulgaris L. var. asperma) is one of the few crops that is only cultivated in eastern parts of Iran. As a new crop there has not been any study to identify phylogenetic relationships of this plant with other related species existing in Iran. In this study, Amplification fragment length polymorphism (AFLP) markers based on four selected primer combinations (EcoRI/Tru1I...

full text

Evaluation of Genetic Diversity in Iranian Violet (Viola spp) Populations Using Morphological and RAPD Molecular Markers

Recognition of genetic reserves and desirable genes is the basis of breeding programs. So far, in Iran, due to the lack of recognition of genetic resources, a considerable breeding program has not been done on native plants. The study of the genetic diversity of violets as a native plant with ornamental and medicinal uses is the great importance in advancing the breeding goals of this plant. So...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 5  issue 2

pages  199- 208

publication date 2018-12-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023